ICS65.020.20 B05DB4401 广州市地方标准 DB4401/T85—2020 代替DB440100/T169—2014 红掌组培种苗生产技术规程 TheproductionregulationofAnthuriumandreanuminplantletvitro 2020-06-08发布 2020-07-01实施 广州市市场监督管理局 发布 DB4401/T85—2020 I目    次 前言.....................................................................................................................................................................Ⅲ 1范围.................................................................................................................................................................1 2规范性引用文件.............................................................................................................................................1 3术语和定义.....................................................................................................................................................1 4设备设施及化学药剂.....................................................................................................................................1 4.1设备设施.................................................................................................................................................1 4.2化学药剂.................................................................................................................................................2 5消毒灭菌.........................................................................................................................................................3 5.1洗涤室消毒灭菌.....................................................................................................................................3 5.2接种室消毒灭菌.....................................................................................................................................3 5.3培养室消毒灭菌.....................................................................................................................................3 5.4超净工作台消毒灭菌.............................................................................................................................3 5.5培养基的消毒灭菌.................................................................................................................................3 5.6接种器具消毒灭菌.................................................................................................................................3 5.7污染瓶苗消毒灭菌.................................................................................................................................3 6培养室环境条件控制.....................................................................................................................................4 6.1温度.........................................................................................................................................................4 6.2湿度.........................................................................................................................................................4 6.3光照.........................................................................................................................................................4 7组培快繁技术.................................................................................................................................................4 7.1培养基配制.............................................................................................................................................4 7.2外植体选择.............................................................................................................................................4 7.3外植体消毒灭菌.....................................................................................................................................4 7.4接种.........................................................................................................................................................5 7.5愈伤组织诱导培养.................................................................................................................................5 7.6愈伤组织增殖培养.